Microfluidic Detection Module For DNA Analysis Using Integrated Microheaters and DNA Microarrays On Plastic Foil
Conference: Sensoren und Messsysteme 2014 - Beiträge der 17. ITG/GMA-Fachtagung
06/03/2014 - 06/04/2014 at Nürnberg, Deutschland
Proceedings: Sensoren und Messsysteme 2014
Pages: 4Language: englishTyp: PDF
Personal VDE Members are entitled to a 10% discount on this title
Authors:
Ohlander, A.; Bauer, S.; Bock, K. (Fraunhofer Research Institution for Modular Solid State Technologies EMFT, Munich, Germany)
Ramachandraiah, H.; Russom, A. (Science for Life Laboratory, KTH Royal Institute of Technology, Sweden)
Bock, K. (TU Berlin, Center for Technologies of Microperipherics, Berlin, Germany)
Abstract:
We report detection of single nucleotide polymorphisms (SNP) by melting curve analysis (MCA) on DNA microarrays in a plastic microfluidic system with integrated heaters using lamination foils. Thin-film copper microheaters were structured on polyethylene napthalate (PEN) foil and DNA microarrays were printed on the heater foil. The system was integrated in a microfluidic channel and successful detection of SNPs was demonstrated. Thermal and electrical characterization of the system was performed proving that the recently implemented low-cost heater encapsulation method using a simple single-sided adhesive foil has not affected the systems performance relative the previous Parylene C encapsulation.