Smartphone-based rapid and highly sensitive immunoassay for human fetuin A
Conference: MikroSystemTechnik 2015 - MikroSystemTechnik Kongress 2015
10/26/2015 - 10/28/2015 at Karlsruhe, Deutschland
Proceedings: MikroSystemTechnik 2015
Pages: 3Language: englishTyp: PDF
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Authors:
Venkatesh, A. G. (Department of Electrical and Computer Engineering, Jacobs School of Engineering, University of California San Diego, San Diego CA 92093, USA)
Stetten, Felix von; Zengerle, Roland; Vashist, Sandeep Kumar (Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany)
Stetten, Felix von; Zengerle, Roland; Vashist, Sandeep Kumar (Laboratory for MEMS Applications, IMTEK – Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany)
Abstract:
A rapid and highly-sensitive smartphone-based immunoassay (SIA) is reported for the detection of human fetuin A (HFA), an important biomarker that needs to be determined in cases of atherosclerosis, diabetes, inflammatory disorders, cardiovascular diseases and other pathophysiological conditions. The developed SIA employs a highly simplified procedure based on covalently bound capture antibody (Ab) and one-step kinetics-based IA. The dynamic range, limit of detection (LOD) and limit of quantification (LOQ) were 0.1-243 ng mL-1, 0.1 ng mL-1 and 0.51 ng mL-1, respectively.