The LabCard – A new approach for centrifugal assay automation
Conference: MikroSystemTechnik 2017 - Kongress
10/23/2017 - 10/25/2017 at München, Deutschland
Proceedings: MikroSystemTechnik 2017
Pages: 3Language: englishTyp: PDF
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Authors:
Rombach, Markus; Keller, M.; Paust, Nils; Stetten, Felix von; Mark, D.; Karle, M. (Hahn-Schickard, Georges-Köhler-Allee 103, 79110 Freiburg, Germany)
Zengerle, Roland (Hahn-Schickard, Georges-Köhler-Allee 103, 79110 Freiburg, Deutschland & IMTEK – Institut für Mikrosystemtechnik, Universität Freiburg, Georges-Köhler-Allee 103, 79110 Freiburg, Deutschland)
Abstract:
We present the LabCard, a new centrifugal microfluidic approach for simultaneous processing of multiple test carriers by arranging them in the vertical plane. As a proof-of-concept, we fluidically integrated an assay for isothermal nucleic acid analyses of respiratory pathogens with the microfluidic network comprising unit operations and processing chains for thermal lysis of a patient sample (25-75 muL), release of pre-stored reagents, mixing of reagents, aliquoting and distribution into amplification wells, which was successfully demonstrated in 5/5 runs with an overall fluidic processing time of 10 min.