Automated particle based C-reactive protein immunoassay with ondisk pre-stored reagents and centrifugo-pneumatic liquid control
Conference: MikroSystemTechnik 2017 - Kongress
10/23/2017 - 10/25/2017 at München, Deutschland
Proceedings: MikroSystemTechnik 2017
Pages: 4Language: englishTyp: PDF
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Authors:
Zhao, Yunpeng; Klein, Vanessa; Czilwik, Gregor (Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany)
Mitsakakis, Konstantinos; Paust, Nils (Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany & Laboratory for MEMS Applications, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany)
Zengerle, Roland (Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany & Laboratory for MEMS Applications, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany & BIOSS-Centre for Biological Signalling Studies, University of Freiburg, 79110 Freiburg, Germany)
Abstract:
We present a fully automated centrifugal microfluidic concept for implementing particle based immunoassay. With all reagents pre-stored on a centrifugal microfluidic LabDisk, the automation is exclusively controlled by the spinning frequency and does not require any additional means. The automated processes include release of secondary antibody solution to incubate with sample and pre-stored functionalized microparticles for one-step immunoassay; release of wash buffer from one stick-pack for two washing steps; and release of substrate and stopping solution to trigger and stop the substrate reaction. The automated immunoassay concept was demonstrated by means of detecting C-reactive protein (CRP) in the range of 1 – 81 ng/ml. The limit of detection and quantification are 1.0 ng/ml and 2.1 ng/ml, respectively.