Microfluidic App for centrifugal separation and purification of lymphatic cancer cells from whole blood
Conference: MikroSystemTechnik 2019 - Kongress
10/28/2019 - 10/30/2019 at Berlin, Deutschland
Proceedings: MikroSystemTechnik Kongress 2019
Pages: 3Language: englishTyp: PDF
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Authors:
Specht, Mara; Burger, Stefan; Rombach, Markus; Paust, Nils; Karle, Marc (Hahn-Schickard, Georges-Köhler-Allee 103, 79110 Freiburg, Germany)
Schemberg, Joerg; Foerster, Tobias; Gastrock, Gunter (Institute for Bioprocessing and Analytical Measurement Techniques, Rosenhof, 37308 Heilbad-Heiligenstadt, Germany)
Zengerle, Roland; von Stetten, Felix (Hahn-Schickard, Georges-Köhler-Allee 103, 79110 Freiburg & Laboratory for MEMS Applications, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Köhler-Allee 103, 79110 Freiburg, Germany)
Abstract:
Hahn-Schickard and the iba Heiligenstadt developed a microfluidic App for separation and purification of target cells from whole blood. Starting from a sample of 2 ml the processing includes plasma separation, density gradient centrifugation and cell separation. All steps are implemented on one centrifugal microfluidic LabDisk that can be processed in a commercial centrifuge. In proof-of-concept experiments the system recovered 90 % of a lymphatic cancer cell type (KG-1) spiked into the sample within 1 h.