BioMEMS for rapid and sensitive capillary immunoassay in hepatitis serology
Conference: Mikrosystemtechnik Kongress 2005 - Mikrosystemtechnik Kongress 2005
10/10/2005 - 10/12/2005 at Munich, Germany
Proceedings: Mikrosystemtechnik Kongress 2005
Pages: 4Language: englishTyp: PDF
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Authors:
Riebeseel, Katja (Institut für Mikrosystemtechnik, IMTEK-Sensoren, Albert-Ludwigs-Universität, Georges-Köhler-Allee 103, 79110 Freiburg, Deutschland )
Enderle, Barbara; Urban, Gerald; Moser, Isabella (Institut für Mikrosystemtechnik, IMTEK-Sensoren, Albert-Ludwigs-Universität, Georges-Köhler-Allee 103, 79110 Freiburg, Deutschland)
Jobst, Gerhard (jobst technologies, Georges-Köhler-Allee 103, 79110 Freiburg, Deutschland)
Abstract:
A foil-based BioMEMS for electrochemical capillary immunoassays in hepatitis serology is presented. The quantification of anti-hepatitis B antibodies in human serum (titer) is important for the proof of a successful vaccination against the disease (immunization status). The application is realized by implementing a sandwich immunoassay into a capillary, in which antibodies (IgGs) against hepatitis B surface antigen (HBs-Ag) are specifically determined. The detection is carried out electrochemically [see Figure 1]. The assay is very sensitive and designed to differentiate between hepatitis-IgG negative serum (< 10 U/L) and positive serum (?? 100 U/L).