Towards cell-based microsystems: Cell-adhesion on polymerized peptide-amphiphilic microstructures

Conference: Mikrosystemtechnik Kongress 2005 - Mikrosystemtechnik Kongress 2005
10/10/2005 - 10/12/2005 at Munich, Germany

Proceedings: Mikrosystemtechnik Kongress 2005

Pages: 4Language: englishTyp: PDF

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Shroff, K.; Rühe, J.; Biesalski, M. (Institute for Microsystems Technology (IMTEK), University of Freiburg, Georges-Köhler-Allee 103, 79110 Freiburg, Germany)

Polymerizable peptide-amphiphiles, i.e. polymerizable fatty acids that are linked to a bioactive peptide headgroup, are prepared and used as molecular building units for the design of 2- and 3-D bioactive materials, which promote the adhesion and spreading of living cells. The versatility of these building blocks is demonstrated with two examples, one where solid supported polymerized peptide-amphiphile monolayers are used to study the adhesion and outgrowth of fibroblast cells, and the other, where polymerized vesicles made from peptideamphiphiles are micro-contact printed on a polymer hydrogel that does not support the attachment of cells. With the latter approach, i.e. a vesicle-based live cell array, we are able to spatially control the outgrowth of model cell-lines.