Effect of miR223 on production of IL-1beta and IL-6 in gingival epithelial cells

Conference: BIBE 2019 - The Third International Conference on Biological Information and Biomedical Engineering
06/20/2019 - 06/22/2019 at Hangzhou, China

Proceedings: BIBE 2019

Pages: 6Language: englishTyp: PDF

Personal VDE Members are entitled to a 10% discount on this title

Wen, Ying; Liu, Binjie (Department of Periodontology, Xiangya stomatology hospital, Central South University, Changsha, China)

The aims of this research are exploring the expression of miR223 when the Porphyromonas gingivalis lipopolysaccharide (Pg. LPS) stimulated human gingival epithelial cells (HGECs); and investigating the effects of miR223 on the levels of interleukin-1beta (IL-1beta) and IL-6 in Pg. LPS-induced HGECs inflammation responses. We established the cell model of periodontitis by Pg. LPS stimulation of HGECs with different concentrations and time. The miR223 was detected by real-time PCR. To determine the effects of miR-223 on the production of IL-1beta and IL-6, HGECs were transfected with a miR-223 expression plasmid and stimulated by Pg. LPS (10mg/L). Levels of NLRP3 mRNA in HEGCs were also investigated by real-time PCR. The expression of IL-1beta and IL-6 were detected by enzyme-linked immunosorbent assay(ELISA). The obtained results showed that miR-223 expression is dramatically reduced in HGECs by Pg. LPS stimulation. The down-regulated miR-223 leads to the increase in the NLRP3 expression and a promotion of IL-6 and IL-1beta production upon Pg. LPS stimulation. However, increased miR-223 expression obviously in HGECs, which in turn suppressed NLRP3 expression and downstream IL-1beta, IL-6 production. Our findings suggest that miR223 might attenuate the inflammatory responses in periodontal tissues.