Integration of Microfluidic Features with Microneedle Electrode Arrays for Intracellular Recording Applications

Konferenz: MikroSystemTechnik - KONGRESS 2009
12.10.2009 - 14.10.2009 in Berlin, Germany

Tagungsband: MikroSystemTechnik

Seiten: 4Sprache: EnglischTyp: PDF

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Held, J.; Gaspar, J.; Ruther, P.; Paul, O. (Department of Microsystems Engineering (IMTEK), University of Freiburg, Germany)
Koester, P. J.; Tautorat, C.; Baumann, W. (Department of Biophysics, University of Rostock, Germany)
Hagner, M. (Department of Physics, University of Konstanz, Germany)
Cismak, A.; Heilmann, A. (Department of Biological Materials and Interfaces, Fraunhofer Institute, Halle, Germany)

The fabrication of hollow microneedle electrodes with fluidic channels arranged in 8 × 8 arrays is reported. Features of the hollow needle electrodes include (i) an increased surface area for improved intracellular potential measurements with simultaneous membrane cell electroporation capabilities, (ii) their potential use in highly parallel patch clamp applications and (iii) the ability to efficiently inject reagents and extract cytoplasm into and from the cell interior, respectively. This paper presents three different fabrication processes to realize hollow microneedle electrode arrays with incorporated microfluidic components, as well as initial experiments with cell cultures.