Microfluidic Detection Module For DNA Analysis Using Integrated Microheaters and DNA Microarrays On Plastic Foil

Konferenz: Sensoren und Messsysteme 2014 - Beiträge der 17. ITG/GMA-Fachtagung
03.06.2014 - 04.06.2014 in Nürnberg, Deutschland

Tagungsband: ITG-Fb. 250: Sensoren und Messsysteme 2014

Seiten: 4Sprache: EnglischTyp: PDF

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Autoren:
Ohlander, A.; Bauer, S.; Bock, K. (Fraunhofer Research Institution for Modular Solid State Technologies EMFT, Munich, Germany)
Ramachandraiah, H.; Russom, A. (Science for Life Laboratory, KTH Royal Institute of Technology, Sweden)
Bock, K. (TU Berlin, Center for Technologies of Microperipherics, Berlin, Germany)

Inhalt:
We report detection of single nucleotide polymorphisms (SNP) by melting curve analysis (MCA) on DNA microarrays in a plastic microfluidic system with integrated heaters using lamination foils. Thin-film copper microheaters were structured on polyethylene napthalate (PEN) foil and DNA microarrays were printed on the heater foil. The system was integrated in a microfluidic channel and successful detection of SNPs was demonstrated. Thermal and electrical characterization of the system was performed proving that the recently implemented low-cost heater encapsulation method using a simple single-sided adhesive foil has not affected the systems performance relative the previous Parylene C encapsulation.