DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer

Konferenz: Sensoren und Messsysteme 2014 - Beiträge der 17. ITG/GMA-Fachtagung
03.06.2014 - 04.06.2014 in Nürnberg, Deutschland

Tagungsband: Sensoren und Messsysteme 2014

Seiten: 4Sprache: EnglischTyp: PDF

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Wu, Chunsheng (Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China)
Bronder, Thomas; Werner, Carl Frederik; Schoening, Michael J. (Institute of Nano- and Biotechnologies, FH Aachen, Campus Juelich, 52428 Juelich, Germany)
Poghossian, Arshak (Research Centre Juelich GmbH, 52425 Juelich, Germany)

Single-stranded deoxyribonucleic acid (ssDNA) probes modified with thiol group were immobilized on the patterned gold surface of a light-addressable potentiometric sensor (LAPS) via gold-thiol bond. The LAPS chip immobilized with ssDNA probe was used as a working electrode in a scanning LAPS measurement system. After the working electrode was incubated with a solution containing strands of complementary target ssDNA, the photocurrent-voltage curves of LAPS shift with increasing target ssDNA concentration. On the contrary, in a control experiment, practically no shift of photocurrent-voltage curve was observed if the LAPS chip was incubated with a solution containing mismatch ssDNA. This provides a convenient and label-free method for the detection of DNA hybridization and therefore, a promising approach for the development of novel LAPS-based DNA chips.