Digital Microfluidics for Microchip-Based Cell Sorting

Konferenz: Mikro-Nano-Integration - 6. GMM-Workshop
05.10.2016 - 06.10.2016 in Duisburg, Deutschland

Tagungsband: GMM-Fb. 86: Mikro-Nano-Integration

Seiten: 5Sprache: EnglischTyp: PDF

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Kahnert, S.; Goehlich, A.; Greifendorf, D.; Vogt, H. (Fraunhofer IMS, Finkenstr. 61, 47057 Duisburg, Germany)
Schreiber, F.; Rennings, A.; Erni, D. (Allgemeine und Theoretische Elektrotechnik (ATE), Universität Duisburg-Essen & CENIDE – Center for Nanointegration Duisburg-Essen, 47048 Duisburg, Germany)
Lennartz, K.; Kirstein, U.; Kueppers, R. (Universitätsklinikum Essen, Institut für Zellbiologie (Tumorforschung), 45147 Essen, Germany)
Bartels, F.; Janzyk, U. (Bartels Mikrotechnik GmbH, Otto-Hahn-Str. 15, 44227 Dortmund, Germany)

Within the framework of the scientific project MINAPSO (Mikrochip Navigierte Parallel Sortieranlage), a device has been developed that is intended to separate specific biological cells out of heterogeneous cell suspensions on chip level. The process relies on parallel splitting of cell containing droplets, accomplished by the effect of electrowetting and recognition of fluorescently labelled cells. We present an automatic cell sorting platform consisting of a silicon based electrowetting chip and a fluorescence microscope. Based on the device, all relevant fluid operations like drop extraction, drop transport and drop splitting were verified by parameter variations. Measurements of particle distributions support the technical feasibility of the sorting concept and the proof of principle is conducted by a complete sorting experiment.